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. 2014 May 8;5(3):66. doi: 10.1186/scrt455

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Copyright © 2014 Zhang and Dou; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Immunofluorescence examination of dewaxed sections of the grafts. Red CM-DiI-labeled cells in a graft section (A) in the nonadherent induction group were visualized only in the interstitial tissue among the seminiferous tubules (B) and showed green fluorophore (C) (merged in (D)) after staining with human insulin antibody under a fluoroscope. A similar phenomenon occurred in a graft section in the adherent induction group (E), (F), (G), (H), whereas CM-DiI-labeled cells in the non-induction control group did not express insulin (I), (J), (K), (L).