Table 1.
Human gene-specific primers and quantitative polymerase chain reaction conditions
| Gene | Primer sequence (5′ to 3′) | Size of PCR product (base pairs) | Annealing temperature (°C) | Cycle number |
|---|---|---|---|---|
| 18SrRNA |
Forward: CGGCTACCACATCCAAGGAA |
187 |
62 |
40 |
| Reverse: GCTGGAATTACCGCGGCT | ||||
| Pdx1 |
Forward: TTCCGGAAGAAAAAGAGCCA |
129 |
62 |
40 |
| Reverse: AAACAGGTCCCAAGGTGGAGT | ||||
| Ngn3 |
Forward: CGCAATCGAATGCACAACCT |
135 |
62 |
40 |
| Reverse: TTGAGTCAGCGCCCAGATGTA | ||||
| Pax4 |
Forward: ATTCAGTGGCCCGTGGAAA |
102 |
62 |
40 |
| Reverse: TCTCTTGCCGACGCCATTT | ||||
| NeuroD1 |
Forward: TCTTTCAAACACGAACCGTCC |
103 |
62 |
40 |
| Reverse: AGATTGATCCGTGGCTTTGG | ||||
| Nkx2.2 |
Forward: GCCACGAATTGACCAAGTGAA |
103 |
62 |
40 |
| Reverse: ATGTCCTTGACCGAAAACCCC | ||||
| Nkx6.1 |
Forward: ACACGAGACCCACTTTTTCCG |
102 |
62 |
40 |
| Reverse: AATAGGCCAAACGAGCCCTCT | ||||
| PCSK1 |
Forward: TCCTCTTTTGCGCTTGGTGT |
139 |
62 |
40 |
| Reverse: TGACCCAAAAGGTCATAGCCC | ||||
| Insulin |
Forward: GCGTGGCATTGTGGAACAA |
123 |
62 |
40 |
| Reverse: CCATCTCTCTCGGTGCAGGA | ||||
| Glucagon |
Forward: ACATTGCCAAACGTCACGATG |
103 |
62 |
40 |
| Reverse: GCAATGAATTCCTTGGCAGCT | ||||
| SST |
Forward: GAAGCAGGAACTGGCCAAGTA |
118 |
62 |
40 |
| Reverse: CCTCATTTCATCCTGCTCAGC | ||||
| PP |
Forward: ACAATGCCACACCAGAGCAGA |
110 |
62 |
40 |
| Reverse: GGCCAGCGTGTCCTCTTTG |
PCR, polymerase chain reaction.