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. 2014 Jul 1;4:5511. doi: 10.1038/srep05511

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(a) LNCaP cells were treated with indicated concentrations cisplatin for 24 hours. Cells were lysed and expressions of androgen receptor (AR) ad prostate-specific antigen (PSA) were determined by immunoblotting assay. (Cropped gel) (b) Data of quantitative immunoblot analysis are expressed as relative density (mean ± SE; n = 3). (c) LNCaP cells were treated with 40 μM of cisplatin with or without R1881 (1 nM) for 24 hours. The conditional media were analyzed by ELISA for PSA concentrations. Each value is presented as % in relation to the control (solvent-treated) group. Data are shown as the mean percentage ± SE (n = 6). (*P < 0.05, **P < 0.01).