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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Curr Protoc Chem Biol. 2012 Sep 1;4:233–247. doi: 10.1002/9780470559277.ch110277

Figure 2.

Figure 2

One-pot three-enzyme synthesis of Lewis x trisaccharide Galβ1–4(Fucα1–3)GlcNAcβProN3 or sialyl Lewis x tetrasaccharide Neu5Acα2–3Galβ1–4(Fucα1–3)GlcNAcβProN3 from L-fucose, ATP, GTP, type II disaccharide Galβ1–4GlcNAcβProN3 or trisaccharide Neu5Acα2–3Galβ1–4GlcNAcβProN3. FKP, a recombinant bifunctional L-fucokinase/GDP-fucose pyrophosphorylase (FKP) from Bacteroides fragilis 9343; PmPpA, Pasteurella multocida inorganic pyrophosphatase. Hp1–3FTΔ66, a recombinant Helicobacter pylori α1–3-fucosyltransferase with a C-terminal 66 amino-acid-residue truncation.