Figure 1.

The localization of dTSPO and its depletion in tspo −/− and dsRNA knockdown flies. (A) The mitochondrial localization of dTSPO in S2 cells. S2 cells were stained with MitoTracker Deep Red (red) and TSPO fluorescent probe (green) sequentially. (B) Western blot of dTSPO, COX-I (IMM), and Porin (VDAC) (OMM) in isolated mitochondria from whole bodies of wild type flies treated with increasing concentrations of digitonin which dissolves the OMM releasing its proteins. dTSPO is lost along with porin. For (1)–(5), the concentrations of digitonin are 0, 0.25, 0.5, 1, 2 mg mL⁻¹. (C) Western blot showing depletion of dTSPO in tspo −/− and dsRNA (RNAi) whole-body knockdown flies (mixture of equal numbers of male and female flies). Porin (VDAC) provides the OMM mitochondrial control and α-tubulin provides the total protein loading control. The densitometry of western films was shown (N = 2). Bars report mean ± SEM.