Figure 3.

Inhibition of apoptosis in isolated larval brain cells by dTSPO inactivation. Effect of H₂O₂ on induction of apoptosis in 3rd-larvae brain cells from tsps +/+ or tspo −/− flies (mixture of equal numbers of male and female flies). (A) Flow cytometry quantification of FITC-Annexin V positive cells (dead cells) versus FITC-Annexin V negative and Propidium Iodide negative cells (healthy) cells (each bar, n = 3–6). (B) Assay of caspase 3/7 activity (n = 3). Bars report mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.