Figure 4.

Effects of dTSPO inhibition on Drosophila lifespan and oxidative stress resistance. (A) Left, exposure of wild type (tspo +/+) male flies to 5 or 50 μm PK11195 (n = 78–88 counted for each group). Right, exposure of tspo +/+ flies to 0.1 or 1 μm Ro5-4864 (n = 62–72 counted for each group). Drugs administered in 4% sucrose at 25 °C. (B) Extension of lifespan of tspo −/− males (left) though not female (right) relative to tspo +/+ flies maintained in standard cornmeal medium at 25 °C, n = 250–350.(C) Extension of lifespan of whole body dTSPO dsRNA knockdown males (left) though not female (right) relative to control flies maintained on cornmeal medium at 25 °C, n = 50–100. (D) Protection of males (left) but not females (right) tspo −/− flies to exposure to 5% H₂O₂ in 5% sucrose/PBS at 25 °C relative to tspo +/+ flies, n = 50–75.