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. 2014 Feb 21;13(3):507–518. doi: 10.1111/acel.12200

Figure 5.

Figure 5

Effect of dTSPO reduction on Aβ42-induced neurodegeneration. Systemic reduction (tspo +/−) or neuron-specific knockdown of dTSPO restored neuronal human Aβ42-induced lifespan reduction in male and female flies. (A) Survival curves of male (left) and female (right) flies in response to neuronal expression of human Aβ42 (elav>Aβ42) relative to control flies (elavGal4 control) lacking the UAS-Aβ42 target gene and amelioration of the lifespan reduction induced by Aβ42 expression by partial systemic inactivation of tspo +/− or neuronal dsRNA inactivation of dTSPO expression (elav>Aβ42, dTSPO-RNAi). (males, n ≈ 200 and females, n ≈ 100). (B, C) Reduced neuronal human Aβ42 induced tissue loss in response to systemic (tspo +/−) or neuronal depletion (dTSPO-RNAi). (B) Representative histological sections for only male brains were displayed. Arrows indicate regions of neuronal loss (vacuole-like), DAE = 45. (C) Quantification of number of vacuole-like regions per single head of male (left, n = 3–6 for each genotype, DAE = 45) or female (right, n = 3–4 for each genotype, DAE = 60) flies. (D) Effect of dTSPO depletion on neuronal Aβ42-induced male fly head caspase 3/7 activation (n = 3), DAE = 20. Each bar reports mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.