Figure 6.

Effect of dTSPO depletion on 7–10 DAE Drosophila mitochondrial OXPHOS. Mitochondria were isolated from whole body homogenates of tspo +/+, tspo +/−, and tspo −/− flies (mixture of equal number of males and females). (A) Mitochondrial oxygen consumption rate when metabolizing site I (pyruvate + malate) substrates in the absence (state 4) or presence (State 3) of ADP. N = 3 for each genotype. (B) Mitochondrial OXPHOS complexes I to V specific activities normalized using citrate synthase activity. N = 3 for each enzyme value. (C) Mitochondrial aconitase activity in male and female tspo +/+ versus −/− flies expressed as the percentage ratio of the endogenous activity divided by the Fe²⁺-recovered activity. N = 3 for each assay. *P < 0.05, ** P < 0.01, *** P < 0.001.