Figure 3.

VZV infection significantly upregulated the transcription factor CREBH. Human fibroblast cells (MRC-5) were grown in tissue culture plates then infected with VZV-32 infected MRC-5 cells or treated with tunicamycin (TM), a N-glycosylation inhibitor. At 72 hpi, RNA was processed as described in legend to Figure 2. All gene transcription measurements were graphed for tunicamycin treated and VZV infected cell samples. (A) By measurements using the UPR specific PCR array, VZV infected cells showed significant upregulation of CREBH with downregulation of PERK and ATF6β. Tunicamycin treatment upregulated to a lesser extent all stress SENSORs. (B) To assess some of the measurements by the UPR array, cDNA from VZV infected and tunicamycin treated cells was submitted for real-time (RT-) PCR using primers specific to CREBH and PERK (see Methods section for primer information). Error bars correspond to standard deviation when averaging.