Figure 6.

VZV infection significantly upregulated the cholesterol synthesis associated transcript INSIG. Human fibroblast cells (MRC-5) were grown in tissue culture plates then infected with VZV-32 infected MRC-5 cells or treated with tunicamycin (TM), a N-glycosylation inhibitor. At 72 hpi, RNA was processed as described in the legend to Figure 2. (A) Transcripts associated with lipid synthesis and metabolism where both VZV infected cells and tunicamycin cells showed similar transcription of RAMP4; VZV infected cells in particular showed more transcription of cholesterol synthesis regulator INSIG. (B) In order to assess two of the lipid transcript measurements by the UPR specific PCR array, RT-PCR was carried out on cDNA from uninfected, tunicamycin treated and VZV infected cells using primers to AMFR/gp78 and INSIG1.