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. 2014 Jul 1;5:307. doi: 10.3389/fimmu.2014.00307

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Copyright © 2014 Gehrmann, Cervello, Montalto, Cappello, Gulino, Knape, Specht and Multhoff.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Representative immunohistochemical images of the HSP70 staining in control liver tissue (CTRL) and in the tissue of a patient with hepatocellular carcinoma (HCC) with an underlying liver cirrhosis (LC). The HSP70 staining intensity was stronger in HCC tissue compared to that of control liver tissue (CTRL) and in areas with liver cirrhosis (LC); left panel 10× magnification, right panel 40× magnification. (B) Semiquantitative analysis of the HSP70 staining intensity in sections of LC-HCC patients (n = 4) at a 10× magnification. The HSP70 staining intensity in the HCC regions ranged from very strong (+++), via intermediate (++) to strong (+); in the LC regions the staining intensity ranged between strong (+), weak (±), and very weak (−) in the four different sections.