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(a) Illustration of naive untreated Ig and β-ME-treated Ig (to destroy disulfide bonds). (b) After treatment with β-ME, Ig in a human serum sample was degraded into light-chain and heavy-chain monomers. The degraded Ig chains in cancer cell lines showed abnormal molecular weights. β-ME, β-mercaptoethanol; Ig, immunoglobulin.
