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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Exp Cell Res. 2014 Mar 31;324(1):30–39. doi: 10.1016/j.yexcr.2014.03.016

Fig. 3.

Fig. 3

AF-ALN is internalized by osteoblast-like cells and co-localizes with RPTPµ. To visualize the uptake of ALN by osteoblastic cells and its co-localization with a phosphatase, ROS 17/2.8 cells were incubated for 30 min with 0.1 µM Alexa Fluor 488-ALN (green), then fixed and incubated overnight with a specific antibody that recognizes RPTPµ, followed by an appropriate dilution of secondary antibody labeled with Alexa Fluor-647 (red). The samples were analyzed by confocal microscopy (scale bar, 31.75 µm). Phase contrast image (A); AF-ALN (B); RPTPµ (C); merge image showing co-localization (arrows) of pixels in yellow (D). (Images A and B are reprinted with kind permission of Elsevier, Archives of Biochemistry and Biophysics, 518 (2012) 95–102, Lezcano, Bellido, Plotkin, Boland and Morelli).