Figure 3.

Effects of sildenafil treatment on skeletal muscle proteome. (A) Skeletal muscle protein synthesis (mean ± SE) after 8 days of treatment, determined using the precursor‐product approach to determine fractional synthesis rate. *p = 0.004 vs. placebo, unpaired t‐test, N = 6 placebo, 5 sildenafil. Canonical (B) and functional (C) pathways differentially affected by sildenafil and placebo, determined using Ingenuity Pathways Analysis (IPA) of protein expression in skeletal muscle biopsy samples (top 6 pathways shown). Canonical (D) and functional (E) pathways differentially affected by sildenafil and placebo, determined using IPA of protein S‐nitrosylation in skeletal muscle biopsy samples (top six pathways shown).