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. Author manuscript; available in PMC: 2015 Mar 19.
Published in final edited form as: Nat Commun. 2014 Mar 19;5:3485. doi: 10.1038/ncomms4485

Figure 1. Endotrophin expression in AT induces fibrosis and inflammation-related genes in WAT.

Figure 1

(a) Q–PCR analysis for stromal–vascular versus adipocyte distribution of Col6 mRNAs. Data represents mean±s.d. (n = 5). **P<0.01 and ***P<0.001 versus stromal-vesicular by two-way analysis of variance. (b) PCR analysis of endotrophin overexpression in different fat pads (EWAT (epididymal), SWAT (subcutaneous), MWAT (mesenteric) and BAT (brown adipose tissue) and other organs in the adiponectin promoter-driven TRE-ETP (AdnP-ETP) transgenic mice and their littermate controls 5 days after Dox induction on a chow diet (n = 5 per group). (c) Immunohistochemical staining with an anti-mouse endotrophin antibody of EWAT from the transgenic mice and their littermate controls. Scale bar, 100 μm. (d) Q–PCR analysis of pro-fibrotic and pro-inflammatory genes affected by endotrophin overexpression in SWAT of the transgenic mice and their littermate controls 5 days after Dox induction (n = 5 per group, Student's t-test for all the P-values).