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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: J Neuroendocrinol. 2014 Jul;26(7):426–438. doi: 10.1111/jne.12163

Fig. 3.

Fig. 3

Effects of a 48-hr and of a 24-hr fast on serum insulin (A, D), serum LH levels (B, E), and on hypothalamic GALP mRNA expression (C, F) in WT and GALP-p110α/β cKO male and female mice, respectively. (A) In males, there was a significant interaction between food manipulation and genotype on serum insulin levels (2-way ANOVA, P < 0.05); multiple comparison test revealed a significant effect of fasting on serum insulin levels in WT animals (* P < 0.05) but not in GALP-p110α/β cKO males (D) A 24 hr fast did not affect serum insulin levels in females of either genotype. (B) A 48 hr fast did not affect serum LH levels in WT or in GALP-p110α/β cKO males and no genotype effect was observed in males subjected to either food manipulation. (E) A 24 hr fast did not affect serum LH levels in female animals of both genotypes. However, compared to WT littermates, GALP-p110α/β cKO females have higher serum LH levels, (2-way ANOVA, repeated measures, P < 0.05). (C) There was a significant genotype effect on hypothalamic GALP mRNA levels in males (2-way ANOVA, **P < 0.01); compared to fed WT mice, GALP mRNA levels from the MBH of GALP-p110α/β cKO males were significantly lower regardless of the food treatment, (Holm-Sidak multiple comparison test, P < 0.05). (F) Hypothalamic GALP mRNA levels were similar in fasted vs. nonfasted females of either genotype. Data were expressed as ± SEM.