Figure 5. Mice vaccinated with exosomes from CFP-treated macrophages were partially protected against a low-dose aerosolized M. tuberculosis infection.

Mice (n=4) were infected with virulent M. tuberculosis H37Rv by aerosol challenge 4 weeks after the final exosome vaccination. Six weeks postinfection, all mice were sacrificed and mycobacterial counts in the lungs (A) and spleens (B) were determined by plating. Results are expressed as the means ± standard errors of 4 mice per group. The data shown is the representative of three independent experiments. *, p < 0.05 compared to results with PBS control groups (one-way ANOVA with Tukey post-test).