Table 1.
Rat-specific primers designed for RT-PCR analysis
| Gene | Accession number | Primer | Tm° | Sequence (5′-3′) |
|---|---|---|---|---|
| GAPDH | NC_005103.3 | GAPDH for | 63.4 | CTCCCATTCTTCCACCTTTG |
| GAPDH rev | 63.8 | ATGTAGGCCATGAGGTCCAC | ||
| IL-1β | NM_031512.2 | IL-1β for | 67.0 | TACCTATGTCTTGCCCGTGGAG |
| IL-1β rev | 67.6 | ATCATCCCACGAGTCACAGAGG | ||
| TNFα | NM_012675.3 | TNFα for | 64.0 | TGCCTCAGCCTCTTCTCATT |
| TNFα rev | 63.2 | GGGCTTGTCACTCGAGTTTT | ||
| IL-6 | NM_012589.2 | IL-6 for | 62.5 | AAAGCCAGAGTCATTCAGAGC |
| IL-6 rev | 63.8 | GAGCATTGGAAGTTGGGGTA | ||
| IL-10 | NM_012854.2 | IL-10 for | 64.0 | CCTGCTCTTACTGGCTGGAG |
| IL-10 rev | 63.8 | TGTCCAGCTGGTCCTTCTTT |