Fig. 1.

Pulmonary levels of IL-17A and IL-22 following inoculation with C. neoformans strain H99γ or H99 and during secondary challenge of protectively and non-protectively immunized mice. BALB/c mice were intranasally inoculated with 1×10⁴ c.f.u. of C. neoformans strain H99 or H99γ (a, b). Alternatively, BALB/c mice received an initial immunization with HK C.n. yeasts or C. neoformans strain H99γ and were allowed 60 days to resolve the infection. Mice then received a second intranasal challenge with 1×10⁴ c.f.u. of C. neoformans strain H99 (c, d). Lung homogenates were prepared from lungs excised on days 1, 3, 5 and 7 post-inoculation and assayed for IL-17A and IL-22 production by ELISA. Data are cumulative of three experiments utilizing four mice per group per time point for each experiment. Asterisks indicate significant differences (*P<0.05).