Fig. 10.

Antimicrobial peptide receptors on macrophages (Macs) and DCs following inoculation of WT and IL-22^−/− mice with H99γ. Mice of 129 (WT) and IL-22^−/− (KO) strains were intranasally inoculated with 1×10⁴ c.f.u. of C. neoformans strain H99γ. Lungs were excised at day 7 post-inoculation and a single cell suspension was generated using enzymic digestion. The leukocytes were evaluated by flow cytometry for the presence of TLR4 on the surface of all leukocytes (a), macrophages (b) or DCs (c) or RAGE on the surface of all leukocytes (d), macrophages (e) or DCs (f). Flow cytometry data are cumulative of two independent experiments using pooled leukocytes from four mice per group per time point for each experiment and are reported as means±sem. Asterisks indicate significant differences (*P<0.05).