Fig. 4.

Antimicrobial peptide receptors on macrophages (Macs) and DCs following inoculation with H99γ compared with H99. BALB/c mice were intranasally inoculated with 1×10⁴ c.f.u. of C. neoformans strain H99 or H99γ. Lungs were excised at days 5 and 7 post-inoculation and a single cell suspension was generated using enzymic digestion. The leukocytes were evaluated by flow cytometry for the presence of TLR4 on the surface of macrophages (a) or DCs (b) or RAGE on the surface of macrophages (c) or DCs (d). Flow cytometry data are representative results of two independent experiments using pooled leukocytes from four mice per group per time point for each experiment.