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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Microbes Infect. 2014 Mar 15;16(6):502–511. doi: 10.1016/j.micinf.2014.02.012

Fig. 3.

Fig. 3

NETs are required for larval killing by human neutrophils in vitro. A) Human macrophages (Mϕ) and neutrophils (PMN) cultured in vitro with 50 larvae (L3) for 48 h. The cultures were treated with 100 U/ml of DNase I to block NET formation and assessed for larval killing. Data are shown as mean ± SD. *, p < 0.001 when compared with all other groups. B) Supernatants from the in vitro assay were analyzed for extracellular DNA using the PicoGreen assay. Data are shown as mean ± SD. *, p < 0.001 compared to L3 alone group or **, p < 0.001 compared to the L3 + PMN + Mϕ group. Each group was performed in triplicate for at least two independent experiments.