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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Microbes Infect. 2014 Mar 15;16(6):502–511. doi: 10.1016/j.micinf.2014.02.012

Fig. 4.

Fig. 4

Mouse NETs are not required for larval killing in vitro. A) Mouse neutrophils (1 × 105) were stimulated ex vivo with 100 larvae (L3) or PMA in the presence of mouse serum as a complement source (C′) or heat inactivated serum for 3, 6, 24 and 48 h. The culture supernatants were analyzed for extracellular DNA using the PicoGreen assay. Data are shown as mean ± SD. B) Mouse bone marrow-derived macrophages (Mϕ) were cultured with neutrophils (PMN) and 50 larvae (L3) for 22 h. The cultures were treated with 100 U/ml of DNase I to degrade NETs and assessed for larval killing. Data are shown as mean ± SD. *, p < 0.01 when compared with wells containing L3 alone. C) Supernatants from the in vitro assay were analyzed for extracellular DNA using the PicoGreen assay. Data are shown as mean ± SD. *, p < 0.001 compared to L3 alone group or **, p < 0.001 when compared to wells with Mϕ and PMN but not treated with DNase I. Each group was performed in duplicate for at least two independent experiments.