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Media exposed to PC-12 pheochromocytoma cells (‘conditioned media’) stimulates proliferation of H508 human colon cancer cells. H508 cell proliferation was determined by absorbance at 490 nm as described in Methods. The proliferative effects of ‘conditioned media’ were significantly attenuated in the presence of 5 μM atropine. Data represent means and standard errors from three independent experiments. At = atropine.
