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BrxA and BrxB are S-bacillithiolated in vivo in CHP-treated cells. FLAG-tagged versions of BrxA, BrxA C55A, BrxA C53A, BrxB, BrxB C54A, and BrxB C52A were expressed in the background of the brxA brxB double mutant. Cells were treated with 50 μM CHP, and samples were collected at the times indicated. The BrxA and BrxB proteins were immunoprecipitated using anti-FLAG antibodies and analyzed by Western blotting with either anti-BSH or anti-FLAG antibodies.
