FIG. 1.

α-l-Iduronidase (IDUA) sequence analysis and oligonucleotide design. (A) Three separate regions of the human IDUA gene were targeted for the development of six nucleases. Above each numerically designated site is its location (intron or exon). The ZiFiT CoDA Design Zinc Finger Nucleases option reveals target sites and spacer sequence and displays the DNA sequence to be assembled. (B) Shown are examples of the nucleotide sequence required to encode a 6-bp left or (C) right array, and a (D) 7-bp left or (E) right ZFN array. Color-coded regions in the left array sequences correspond to Finger One (green), Finger Two (pink), and Finger Three (orange). In the right arrays Finger One is in blue, Finger Two is in green, and Finger Three is in red.