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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: J Immunol Methods. 2014 Apr 13;407:120–126. doi: 10.1016/j.jim.2014.04.003

Fig. 5.

Fig. 5

Comparative protein yields of exosomes isolated with acetate vs. 100,000g ultracentrifugation. A) 50 mL of pre-cleared cell supernatant was centrifuged at 100,000g for 1 hr or at 5,000g for 10 min. after 60 min incubation with acetate. The pellets were resuspended in HBS and protein was quantified. A second round of centrifugation and acetate treatment was carried out with the entire supernatant from the acetate treatment and ultracentrifugation, respectively. Flow analysis of exosomes stained with (B), alix antibodies and (C) FITC-annexin 5.