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. 2014 Jun 4;4(6):140027. doi: 10.1098/rsob.140027

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© 2014 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 2. — General view of AND, PAR and CTR placentae at day 20. (a) Similar size and gross morphology of AND, PAR and CTR placentae at day 20 of development (left column). Histological differences in development of AND, PAR and CTR placentae: a vascular network (indicated by arrowheads in the right column) was present in the allantois of PAR and CTR placentae, while completely absent in AND placentae. Trophoblastic cells of columnar shape with intense cytoplasmic vacuolization were evident in AND placentae, while PAR placentae had a low number of enlarged trophoblastic cells (trophoblastic layer indicated by arrow and delimited by dashed line). (b) Severe cellular death in PAR placentae revealed by TUNEL analysis of histological sections (***p < 0.001).