Figure 2.

miR-17 versus miR-20a differential effect on ADAR 3′UTR. (a) Dual luciferase assay conducted on ADAR 3′UTR as representative target gene. The assay included forced expression of miR-17 and miR-20a in HEK 293t cell line. The miRNAs transfected cells were separately co-transfected with the dual luciferase psicheck2 vector included the mutated 3′UTR (red bars) of interest compared with the wild-type 3′UTR (black bars). The upstream Renilla luciferase activity was measured and normalized to the firefly constitutive luciferase activity. Asterisk denotes p-value of <0.05. (b) Verification of miR-17-5p and miR-20a over-expression in HEK 293t transfectants, as compared with scrambled-transfected cells. The y-axis denotes fold change above scrambled-transfected cells (black bars, control; red bars, over-expression).