Figure 4.

Phase diagram of lipidic cubic phase use Monoolein. A) A representative figure of GPCR in bicelle. The receptor is surrounded by a DMPC lipid bilayer, which is further covered by detergents. The receptor-lipid-detergent is afloat in the solution. D) the phase-temp diagram of Monoolein and water system⁷², the phase will transit as lipid:water ratio or temperature change; C) crystallization of GPCRs in cubic phase, the receptor is in cartoon, the bicontinuous lipid bilayer is drawn in yellow and white and the dark blue represents the water channel and aqueous solution; D) Phase behavior of lipid-water-PEG400 system, representing the influences of additives and lipids on the phase⁸¹.