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. 2014 Jun 19;13:88. doi: 10.1186/1475-2859-13-88

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Copyright © 2014 Borrero-de Acuña et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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SDS-PAGE gels demonstrating the recombinant production of pyruvate dehydrogenase and 6-phosphogluconolactonase by the recombinant P. putida strains. (A) Shown the separations of whole cell protein extracts from KT2440 (lane 1), KT2440-acoA (lane 2), KT2440-pgl (lane 3), and (B) KT2440Δgcd (lane 4), KT2440Δgcd-acoA (lane 5) and KT2440Δgcd-pgl (lane 6).