Table 2.
Monomer composition of mcl-PHA produced by metabolically engineered P. putida strains in flask experiments
| Strain | C6 ∞ | C8 ∞ | C10 ∞ | C12 ∞ | C12:1 ∞ | C14 ∞ |
|---|---|---|---|---|---|---|
|
KT2440 |
n.d. |
11.8 ± 0.2 |
73.0 ± 0.1 |
4.7 ± 0.2 |
9.4 ± 0.7 |
0.8 ± 0.3 |
|
KT2440Δ
gcd
|
n.d. |
14.0 ± 0.4 |
71.1 ± 0.8 |
4.6 ± 0.9 |
8.5 ± 0.4 |
1.1 ± 0.2 |
|
KT2440-
acoA
|
n.d. |
13.0 ± 0.6 |
69.1 ± 1.2 |
3.4 ± 0.7 |
10.5 ± 0.2 |
3.9 ± 0.2 |
|
KT2440Δ
gcd-acoA
|
n.d. |
14.8 ± 0.7 |
70.2 ± 1.4 |
5.1 ± 0.3 |
8.8 ± 0.1 |
0.7 ± 0.3 |
|
KT2440-pgl |
n.d. |
12.2 ± 0.9 |
74.1 ± 1.5 |
3.8 ± 0.5 |
9.9 ± 0.8 |
n.d. |
| KT2440Δ gcd-pgl | n.d. | 14.6 ± 0.3 | 73.1 ± 0.8 | 3.7 ± 0.2 | 8.1 ± 0.5 | 0.5 ± 0.1 |
n.d.: not detected, less than 0.2%.
∞: The data were determined by GC/MS and are given as relative molar fraction (%) of C6: 3-hydroxyexanoate, C8: 3-hydroxyoctanoate, C10:3-hydroxydecanoate, C12: 3-hydroxydodecanoate, C12:1: 3-hydroxy-5-cis-dodecanoate, and C14: 3-hydroxytetradecanoate.