Table 4.
Expression profile of the genes belonging to PHA biosynthesis and central metabolic pathways in the metabolically engineered P. putida strains compared to their parental strains
|
Gene name |
Locus tag |
Description |
Fold change
§
|
|
|---|---|---|---|---|
| Δ gcd-acoA | KT2440-acoA | |||
|
PHA synthesis |
|
|
|
|
|
phaI |
PP5008 |
PHA granule-associated |
0.7 |
0.9 |
|
phaF |
PP5007 |
PHA granule-associated |
-0.3 |
0.2 |
|
phaC1 |
PP5003 |
PHA polymerase |
-0.2 |
0.7 |
|
phaC2 |
PP5005 |
PHA polymerase |
-0.6 |
-0.2 |
|
phaZ |
PP5004 |
PHA depolymerase |
0.3 |
0.5 |
|
phaD |
PP5006 |
Transcriptional regulator |
-0.4 |
0.3 |
|
phaG |
PP1408 |
Acyl-transferase |
1.5 |
-0.1 |
|
Transporters |
|
|
|
|
|
oprB-1 |
PP1019 |
Porin |
-0.7 |
-1.0 |
|
oprB-2 |
PP1445 |
Porin |
-0.5 |
-0.6 |
|
Glycolysis/gluconeogenesis |
|
|
|
|
|
glk |
PP1011 |
Glucokinase |
0.2 |
-0.2 |
|
pgi |
PP1808 |
Glucose-6-phosphate isomerase |
-0.5 |
0.0 |
|
fbp |
PP5040 |
Fructose-1,6-bisphosphatase |
0.6 |
0.4 |
|
fda |
PP4960 |
Fructose-1,6-bisphosphate aldolase |
0.9 |
0.1 |
|
tpiA |
PP4715 |
Triosephosphate isomerase |
0.8 |
0.6 |
|
gap1 |
PP1009 |
GAP dehydrogenase, type I |
-1.2 |
-0.4 |
|
gap2 |
PP2149 |
GAP dehydrogenase, type II |
0.2 |
0.3 |
|
pgk |
PP4963 |
Phosphoglycerate kinase |
0.2 |
0.1 |
|
pgm |
PP5056 |
Phosphoglyceromutase |
0.4 |
0.1 |
|
eno |
PP1612 |
Phosphopyruvate hydratase |
0.3 |
0.0 |
|
pyk |
PP1362 |
Pyruvate kinase |
-0.3 |
0.4 |
|
Pentose phosphate pathways |
|
|
|
|
|
zwf1 |
PP1022 |
G6P dehydrogenase |
0.9 |
0.2 |
|
zwf2 |
PP4042 |
|
0.1 |
-0.3 |
|
zwf3 |
PP5351 |
|
0.2 |
0.4 |
|
pgl |
PP1023 |
6-phosphogluconate dehydrogenase |
0.5 |
-0.9 |
|
gnd |
PP4043 |
6-phosphoglucolacto dehydrogenase |
-0.6 |
-0.1 |
|
gnuK |
PP3416 |
Carbohydrate kinase |
-1.1 |
-0.6 |
|
kguK |
PP3378 |
Dehydroglucokinase |
0.45 |
-0.4 |
|
kguD |
PP3376 |
2-Ketogluconate 6-phosphate reductase |
-0.7 |
-0.5 |
|
rpiA |
PP5150 |
Ribose-5-phosphate isomerase A |
0.3 |
0.0 |
|
rpe |
PP0415 |
Ribulose-phosphate 3-epimerase |
-0.5 |
-0.1 |
|
tktA |
PP4965 |
Transketolase |
0.3 |
0.7 |
|
tal |
PP2168 |
Transaldolase B |
0.4 |
0.3 |
|
Entner-Doudoroff pathway |
|
|
|
|
|
edd |
PP1010 |
6-Phosphogluconate dehydratase |
-0.3 |
-0.3 |
|
eda |
PP1024 |
KDPG aldolase |
0.0 |
-0.5 |
|
Pyruvate metabolism |
|
|
|
|
|
acoA |
PP0555 |
Pyruvate dehydrogenase |
12.4 |
5.3 |
|
acoB |
PP0554 |
Pyruvate dehydrogenase |
1.5 |
1.0 |
|
acoC |
PP0553 |
Pyruvate dehydrogenase |
0.7 |
0.7 |
| |
PP0545 |
Aldehyde dehydrogenase |
0.1 |
-1.0 |
|
acsA |
PP4487 |
Acetyl-CoA synthetase |
0.8 |
1.4 |
|
accC-2 |
PP5347 |
Pyruvate carboxylase |
0.5 |
0.2 |
|
ppsA |
PP2082 |
Phosphoenolpyruvate synthase |
0.5 |
0.4 |
|
ppc |
PP1505 |
Phosphoenolpyruvate carboxylase |
0.5 |
0.4 |
|
TCA cycle |
|
|
|
|
|
gltA |
PP4194 |
Citrate synthase |
0.7 |
0.7 |
|
acnA |
PP2112 |
Aconitate hydratase |
0.4 |
-0.4 |
|
acnB |
PP2339 |
Aconitate hydratase |
0.7 |
0.3 |
|
icd |
PP4011 |
Isocitrate dehydrogenase |
0.3 |
-0.5 |
|
sucA |
PP4189 |
2-Oxoglutarate dehydrogenase |
-0.7 |
-0.2 |
|
sucD |
PP4185 |
Succinyl-CoA synthetase sub alpha |
-0.1 |
-0.3 |
|
sucC |
PP4186 |
Succinyl-CoA synthetase sub beta |
0.2 |
0.3 |
|
sdhA |
PP4191 |
Succinate dehydrogenase |
0.3 |
0.4 |
|
fumC |
PP0944 |
Fumarate hydratase |
0.5 |
-1.1 |
|
mdh |
PP0654 |
Malate dehydrogenase |
-0.4 |
-1.3 |
|
Glyoxylate shunt |
|
|
|
|
|
aceA |
PP4116 |
Isocitrate lyase |
2.9 |
1.3 |
| glcB | PP0356 | Malate synthase | 0.8 | 0.8 |
§Bold numbers represent a differentiated gene expression pattern.