Table 6.
Levels of the NAD + , NADH, NADP + , and NADPH cofactors and their ratios of various P. putida strains under PHA producing conditions
|
Strain |
|
Cofactor level (mmol/g rCDW)* |
Cofactor ratio |
|||
|---|---|---|---|---|---|---|
| NAD + | NADH | NADP + | NADPH | NADH/NAD + | NADPH/NADP + | |
| KT2440 |
7.62 ± 0.86 |
0.67 ± 0.08 |
3.22 ± 0.38 |
2.67 ± 0.07 |
0.09 |
0.83 |
| KT2440Δgcd |
6.01 ± 0.67 |
1.75 ± 0.36 |
4.49 ± 0.12 |
1.31 ± 0.32 |
0.30 |
0.29 |
| KT2440-acoA |
6.25 ± 0.42 |
2.02 ± 0.15 |
6.48 ± 0.48 |
0.87 ± 0.27 |
0.32 |
0.13 |
| KT2440Δgcd-acoA |
6.88 ± 0.33 |
2.94 ± 0.15 |
3.98 ± 0.46 |
0.96 ± 0.36 |
0.43 |
0.24 |
| KT2440-pgl |
5.59 ± 0.14 |
1.82 ± 0.10 |
2.58 ± 0.40 |
3.07 ± 0.41 |
0.33 |
1.19 |
| KT2440Δgcd-pgl | 10.81 ± 0.50 | 2.18 ± 0.38 | 2.30 ± 0.15 | 3.71 ± 1.23 | 0.20 | 1.62 |
*rCDW: residual cell dry weight.