A. Gating strategy to determine antibodies bound per cell (ABC) values of CD5 in CD45+/CD19+ B cells. This specimen is peripheral blood of a normal donor and the CD19+ cells show a variable level of CD5 expression ranging from negative to positive. This is designated as a heterogeneous pattern of CD5 expression. B. This dot plot of a peripheral blood specimen of a patient with chronic lymphocytic leukemia (CLL) displays CD19+ cells with a high expression level of CD5, with a compact distribution, designated as a homogeneous positive pattern. C. This dot plot of a case of mantle cell lymphoma displays a homogeneous positive pattern of CD5 expression. D. This case of marginal zone lymphoma (MZL) displays a heterogeneous pattern of CD5 expression. E. This case of follicular lymphoma displays dim reactivity with CD19, and a dim/negative pattern of CD5 expression.
[The y-axis logarithmic numbers of figures 1A–1E refer to antibodies bound per cell].