Table 1.
Primer sequences used for CsCCD7 and CsCCD8 genes cloning and analysis
| Sequences | Primers (5′-3′) | |
|---|---|---|
| Degenerate primers |
CCD8-F0 |
GTSGTGAGRATGGAASCHGG |
| CCD8-F1 |
GNCAYYTNTTYGYGGNTAYGC |
|
| CCD8-F2 |
CNCCNYTNTAYAARTTYARTGGCA |
|
| CCD8-F3 |
ATMCCAYTKGAYGGRAGC |
|
| CCD8-R0 |
CCATCATCYTCWTSGGTGC |
|
| CCD8-R1 |
CCAYTRAAYTTRTANARNGGNGT |
|
| CCD8-R2 |
TNGTNARNGTRTTNGGRAARCART |
|
| CCD8-R3 |
CCARCADCCRTGVARGCC |
|
| CCD7-F0 |
GACGAYCAYGGYTCCACSGTSCAC |
|
| CCD7-F1 |
CTCGACGGCCAYGGYTACCT |
|
| CCD7-R1 |
CYCKRTGCGTRAACCRCCACGA |
|
| Genome walker |
CCD8-F1 |
GGTGTGGTTATSTCTGTGATAAGTG |
| CCD8-F2 |
CCAACTTCGAGGAGTTGCACGA |
|
| CCD8-R1 |
ATGCTCATTCGGGTCGAGTGCT |
|
| CCD8-R2 |
CTTTGTCGCTCCCATCCAATGGT |
|
| CCD8-R3 |
TTGCATGGCCTCACAGCTCCA |
|
| CCD8-R4 |
CAACTCTCCTTTGTCGCTCCCATC |
|
| CCD8-R5 |
TGCCGTTGATGAAGTGGAAGGTCAG |
|
| CCD8-R6 |
TGTTGGCGTTGTCGCTGAGTGAC |
|
| CCD8-R7 |
AGAAGTTGTCGGTCTTCGGGACT |
|
| CCD8-R8 |
TACATGCTAGCCACAAGGGCTCCA |
|
| CCD8-R9 |
TGGTCTGGCGTTCGGTAGGTGCT |
|
| CCD7-F3 |
TCTCGGTGAACCCAAGCCAGCA |
|
| CCD7-F4 |
GCAGACATCACCCATATATCTGCT |
|
| CCD7-F5 |
TCTCGGTGAACCCAAGCCAGCA |
|
| CCD7-R1 |
CAAGACAAAAAGGAATGGCA |
|
| CCD7-R2 |
CAGTCGTGGATCATCAACTGGTCTG |
|
| CCD7-R3 |
TGTCTCCATCTGTTGTCTGGTTG |
|
| CCD7-R4 |
ATGATGGAGCTTATGTTGGGTCA |
|
| cDNA isolation |
CCD7-F |
ATGCACTCCATTTCTCACCGC |
| CCD7-R |
TAGTGATTTCTGCGAGACCA |
|
| CCD8a-F |
ATGGCAGACGTAGGGATACTGA |
|
| CCD8b-F |
CATGCAAACAAACTTAATAGCT |
|
| CCD8-R |
CTTGTCCTCTATTAAATTACTTC |
|
| QRT-PCR expression analysis |
CCD7-F |
ACCTCCCCGTCATCCAAT |
| CCD7-R |
ATGACGGTTTCGGTCTCG |
|
| CCD8-F |
GAAGGGGTCGATCGAGGT |
|
| CCD8-R | CGTCGCCGTACTCGAACT |