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. 2014 Jun 19;8:14. doi: 10.1186/1754-1611-8-14

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Copyright © 2014 Brie et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Immunocytochemical staining for osteocalcin Texas red, counterstained with DAPI in osteoblasts cultured in complete medium on titanium implants evaluated after 28 days of cultivation. Legend: non-infiltrated, control Ti (Ti control), titanium infiltrated with hydroxyapatite (Ti HA), Ti infiltrated with silicatitanate (Ti SiO₂) (magnification 100×).