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. 2014 Jun 19;8:14. doi: 10.1186/1754-1611-8-14

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Copyright © 2014 Brie et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Levels of osteopontin (OP) secreted in the culture medium by osteoblasts cultured on the implants’ surface. A. in medium wih fetal calf serum (FCS), collected on day 8, 14, 21 and 28 (analysis with two-way ANOVA Bonferroni posttest *p < 0.05; **p < 0.01; ***p < 0.001); B. in serum-free medium (collected on day 21 and 28), in simple (OS) and in complex (OC) differentiation mediums (collected on day 35). Legend: cells cultivated in plastic dishes (ctrl), non-infiltrated, control Ti (Ti control), titanium infiltrated with hydroxyapatite (Ti HA), Ti infiltrated with silicatitanate (Ti SiO₂).