Table 1.
Carbonate chemistry of the pCO2 acclimations at the time of harvesting and in cell-free media (reference); Attained pCO2, DIC, HCO3 −, CO3 2−, and Ωcalcite are calculated based on measured pHNBS and TA using CO2sys (Pierrot et al. 2006)
| Strain, ploidy | Treatment pCO2 (μatm) | Attained pCO2 (μatm) | pHNBS | TA (μmol kg−1) | DIC (μmol kg−1) | CO2 (μmol kg−1) | HCO3 − (μmol kg−1) | CO3 2− (μmol kg−1) | Ωcalcite |
|---|---|---|---|---|---|---|---|---|---|
| RCC 1216, 2N | Low, 380 | 353 ± 8 | 8.19 ± 0.02 | 2,259 ± 19 | 2,023 ± 15 | 13 ± 0 | 1,857 ± 13 | 161 ± 3 | 3.9 ± 0.1 |
| High, 950 | 847 ± 55 | 7.86 ± 0.04 | 2,278 ± 20 | 2,156 ± 2 | 32 ± 2 | 2,060 ± 28 | 84 ± 4 | 2.0 ± 0.1 | |
| RCC 1217, 1N | Low, 380 | 345 ± 4 | 8.23 ± 0.00 | 2,317 ± 12 | 2,068 ± 10 | 13 ± 0 | 1,885 ± 10 | 170 ± 1 | 4.1 ± 0.0 |
| High, 950 | 837 ± 25 | 7.89 ± 0.01 | 2,317 ± 3 | 2,210 ± 5 | 32 ± 1 | 2,092 ± 5 | 86 ± 3 | 2.1 ± 0.1 | |
| Cell-free medium | Low, 380 | 405 ± 3 | 8.17 ± 0.00 | 2,304 ± 5 | 2,092 ± 5 | 15 ± 0 | 1,926 ± 5 | 151 ± 1 | 3.7 ± 0.0 |
| High, 950 | 997 ± 17 | 7.82 ± 0.01 | 2,305 ± 7 | 2,214 ± 12 | 38 ± 1 | 2,128 ± 11 | 75 ± 1 | 1.8 ± 0.0 |
Results are reported for 15 °C (n ≥ 3; ± SD)