Table 1.
Primary Antibodies Used
| Antigen | Immunogen | Manufacturer | Dilution |
|---|---|---|---|
| Toll-like receptor 3 | A mix of synthetic peptides correponding to amino acids 135–150 (SIHKIKSNPFKNQKNL), 828–844 (CRRFKVHHAVQQAIEQN), and 876–891 (CILNWPVQKERINAFH) of mouse TLR3 | Abcam (Cambridge, UK), rabbit polyclonal, #ab53424 | 1:200 |
| phospho-NF-KB p65 (Ser 276) | A synthetic phosphopeptide corresponding to residues surrounding Ser 276 of human NF-KB p65 | Cell Signaling Technology (Danvers, MA, USA), rabbit polyclonial, #3037 | 1:400 |
| Cleaved caspase-3 | A synthetic KLH-coupled peptide (CRGTELDCGIETD) adjacent to Asp 175 in human caspase-3 | Cell Signaling Technology (Danvers), rabbit polyclonial, #9661 | 1:400 |
| Olfactory marker protein (OMP) | Purified rodent OMP | Wako chemical USA (Richmond, VA, USA), goat polyclonal, #544-10001 | 1:8,000 |
| Beta III tubulin (βIIIT) | A synthetic pepetide corresponding to amino acids 443–450 (ESESQGPK) of human class III beta-tubulin | Millipore (Billerica, MA, USA), mouse monoclonal, #MAB1637 | 1:400 |
| Ki67 | 22-amino-acid ki67 repeat motif (APKEKAQPLEDLASFQELSQ) | BD Bioscience (San Jose, CA, USA), mouse monoclonal (Clone B56), #550609 | 1:400 |
| Neutrophil | Highly purified BALB/c mouse neutrophils | Abcam (Cambridge, UK), rat monoclonal (Clone NIMP-R14), #ab2557 | 1:400 |
| F4/80 | Mouse thioglycollate-stimulated peritoneal macrophages | AbD Serotec (Oxford, UK), rat monoclonal (Clone CI:A3-1), #MCA497 | 1:100 |
| CD3 | A synthetic peptide spanning amino acids 156–168 of the cytoplasmic domain of human CD3ε chain | Nichirei Corporation (Tokyo, Japan), rabbit monoclonal (Clone SP7), #413601 | 1:400 |
| Phospho-IRF-3 (Ser 396) | A Synthetic phosphopeptide corresponding to residues surrounding Ser 396 of human IRF-3 | Cell Signaling Technology (Danvers), rabbit monoclonal, #4947 | 1:2,000 |
| β-actin | A KLH conjugated synthetic peptide corresponding to N-terminus of β-actin | MBL (Nagoya, Japan), rabbit polyclonal, #PM053 | 1:10,000 |