Table 4.
Oligonucleotide primers used in this study
| Primer name | Sequence |
|---|---|
| Cloning |
|
| AttbPc12g07500-RV |
GGGGACAAGTTTGTACAAAAAAGCAGGCTATGATTTGGAAATCTCTCTTTAGTGCTTTGGCC |
| AttbPc12g07500-RV |
GGGGACCACTTTGTACAAGAAAGCTGGGTTCACATGCTACCTCTAGCC |
| AttbMF(Alpha)1-FW |
GGGGACAAGTTTGTACAAAAAAGCAGGCTATGAGATTTCCTTCAATTTTTACTGC |
| MF(Alpha)1 Spe1-RV |
GGCTAGCACTAGTGTAAGCTTCAGCCTCTCTTTTCTCG |
| Pc12g07500 Spe1-FW |
CGTTAGACTAGTGCCCCAGCTCTCGATTCAGAGC |
| Expression (RT-qPCR) |
|
| Pc12g07500 208 FW |
TCCGGGTACCTTGACTGTGC |
| Pc12g07500 208 RW |
CCCGAGCCACCTTGAAAGAC |
| Pc12g07500 209 FW |
TCCGGGTACCTTGACTGTGC |
| Pc12g07500 209 RV |
CCCGAGCCACCTTGAAAGAC |
| Pc12g13330 210 FW |
GACGCTTTGCAACCAGAAGG |
| Pc12g13330 210 RV |
TGGGACGGTACCCAAAGATG |
| 3′ FW ACT1 |
GGCTTCTTTGACTACCTTCCA |
| 3′ RV ACT1 | AGAAACACTTGTGGTGAACGA |