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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Gynecol Oncol. 2014 Apr;133(1):83–89. doi: 10.1016/j.ygyno.2013.12.006

Figure 2. CGRRF1 increases the sensitivity of Ishikawa cells to metformin therapy and modulated endometrial cell proliferation.

Figure 2

(A) Ishikawa cells were transfected with a pCMV6/CGRRF1 expression construct (Cg) or control vector (Co) and stable clones were isolated. Cells were then treated with 10 mM metformin for 48 hours, and cell proliferation evaluated by MTT. * p<0.001 control vs. metformin; # p<0.001, Ishikawa (Co) vs. Ishikawa (Cg). (B) ECC-1 cells were transiently transfected with either control or CGRRF1 siRNA. 24 hours following transfection, MTT assays were performed as a measure of cell proliferation. A reduction in CGRRF1 expression is associated with an increased proliferative rate. * p<0.05 control vs. CGRRF1 siRNA.