Table 1.

Comparison of galactolipid and phospholipid changes as affected by added N and nodulation. Lipids were extracted from two week old soybean leaves and roots grown under nitrogen sufficient (5 mM) and deficient conditions (0.5 mM).

	Leaf
	Nitrogen				Rhizobium
	5 mM	SD	0.5 mM	SD	+	SD	-	SD
MGDG/DGDG (98–113/70–85)	2.16	0.13	1.9	0.11	2.30	0.14	2.18	0.19
DGDG/PC (70–85/1–20)	3.19	0.36	1.71	0.19	3.10	0.15	1.74	0.26
DGDG/PE (70–85/21–43)	8.99	1.06	4.73	0.51	9.45	0.64	4.78	0.91
GL/PL (70–85; 98–126)/(1–69,86–97,127–156)	4.75	0.56	2.55	0.30	4.78	0.28	3.02	0.45
	Root
	Nitrogen				Rhizobium
	5 mM	SD	0.5 mM	SD	+	SD	-	SD
MGDG/DGDG (98–113/70–85)	1.0	0.07	1.5	0.15	1.96	0.10	1.50	0.19
DGDG/PC 70–85/1–20)	0.12	0.01	0.06	0.002	0.07	0.002	0.11	0.01
DGDG/PE (70–85/21–43)	0.19	0.01	0.10	0.01	0.10	0.01	0.14	0.02
GL/PL (70–85; 98–126)/(1–69,86–97,127–156)	0.11	0.01	0.07	0.01	0.10	0.01	0.11	0.01

The values are the average of 15 replicates of three independent experiments with ±SD. For the nodulation experiment, four week old leaves and roots were used for lipid extraction. The values are average of 5 replicates with ±SD. ESI-MS/MS was used to analyze the lipids. GL, galactolipids; PL, phospholipids