Figure 4.

Secretory immunoglobulin A (sIgA) in vaginal washes from immunized mice. Vaginal washes were collected on 14, 28, and 42 days after first administration and mZP3-specific sIgA Ab was determined by ELISA. Secretory IgA level of group coadministered with Chi-p-GM-CSF + Chi-(p-mZP3 + mZP3) was significantly higher than other groups (*P < .05; **P < .01; ***P < .001). ELISA indicates enzyme-linked immunosorbent assay; GM-CSF, granulocyte–macrophage colony-stimulating factor; Ab, antibody; mZP3, mouse zona pellucida 3.