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Comparison of geraniol (1) and nerol (2) oxidation catalysed by CYP154E1 wild type and three variants.
| Product distribution: Peak areaa | Conversion | |||||
| geraniol (1) | nerol (2) | 1 | 2 | |||
| CYP154E1 | 3 (100%) | 4 (96%) | 6 (4%) | 98% | 77% | |
| V286L | 5 (26%) | 7 (74%) | 8 (42%) | 6 (58%) | 11% | 4% |
| V286A | 5 (59%) | 7 (41%) | 8 (49%) | 6 (51%) | 6% | 3% |
| V286F | 5 (58%) | 7 (42%) | 8 (52%) | 6 (48%) | 45% | 22% |
aDetermined by GC–MS.
