Table 2.
Comparison of geranylacetone (9) and nerylacetone (10) oxidation catalysed by CYP154E1 wild type and three variants.
| Product distribution: Peak areaa | Conversion | |||
| geranylacetone (9) | nerylacetone (10) | 9 | 10 | |
| CYP154E1 |
11 (8%) 13 (41%) 15 (42%) 17 (9%) |
12 (12%) 14 (32%) 16 (56%) |
98% | 97% |
| V286L |
11 (44%) 13 (18%) 15 (35%) 17 (3%) |
12 (28%) 16 (72%) |
20% | 19% |
| V286A |
11 (46%) 13 (16%) 15 (15%) 17 (23%) |
12 (25%) 16 (75%) |
25% | 42% |
| V286F |
11 (60%) 13 (9%) 15 (31%) |
12 (24%) 14(13%) 16 (63%) |
75% | 90% |
aDetermined by GC–MS.