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Representative images of CFP channel, FRET channel, and pseudocolored emission ratio (Y/C) in each condition [blue (L), low emission ratio; red (H), high emission ratio]. Scale bar is 10 µm. A summary graph showing average of emission ratio (Y/C) in each condition (n = number of cells) (****p<.0001, one-way ANOVA, uncorrected Fisher's LSD).
