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By adding osteopontin into the collagen I hydrogel coating we were able to demonstrate the ability to customize the invasion assay matrix. When osteopontin was added the percentage of C4-2Bs in monoculture with protrusions increased to the level typically observed by C4-2Bs in coculture with MC3T3-E1s. Data was collected from 3 independent experiments with 8 channels per condition. Errors bars represent standard deviation.
