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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Free Radic Biol Med. 2014 Apr 16;72:247–256. doi: 10.1016/j.freeradbiomed.2014.04.011

Figure 4. Effects of EC supplementation on epididymal adipose and liver tissue NOX upregulation.

Figure 4

A,B: Protein levels of NOX subunits (NOX2, NOX4, p47) were measured by Western blot in A- adipose tissue and B- liver, and bands were quantified. B- NOX activity was measured in liver as described in methods. Results for the HFr (HF) and HFr + EC (EC) were referred to control group values (C). C- NOX2 and NOX4 mRNA were measured by quantitative real-time PCR, normalized against TATA-Box binding protein (TBP) and were referred to control group values (C). D- Protein carbonyls were measured as described in methods. Results are shown as mean ± SEM of 4-8 animals/treatment. *Significantly different from other groups (p<0.05, one way ANOVA test).